Nikon Ti2 CSU-W1 Spinning Disk

Available Lasers

Laser Power at x20 Objective
405nm 2.5mW
488nm 6.0mW
561nm 7.15mW
633nm 9.0mW

Available Filter Sets

Fluorophore Excitation Dichroic Emission
DAPI 405nm Quad or Triple 447/60nm
eGFP 488nm Quad or Triple 525/50nm or 525/30nm
Cy3 561nm Quad or Triple 600/52nm or 607/70
Texas Red 561nm (~50% efficient) Quad or Triple 630/92nm or 647LP
Cy5 633nm Quad 708/75nm or 647LP

Software Modules

  • Nikon Elements 5.11 (Also available offline)
  • Nikon Jobs

Objectives

Type Magnification Correction NA Contrast Method
Plan Apo VC x100 Oil 1.4  
Plan Apo Lambda x60 Oil 1.4  
Plan Apo VC x60 Water 1.2 DIC
Plan Apo VC x20 Air 0.8 DIC
Plan Apo Lambda x10 Air 0.5  

Supporting Equipment

  • Cherry Temperature Controller 5-45ºC in 10sec shifts
  • Bruker FRAP module
  • Slide Holder
  • 35mm Petri Dish Holder
  • Multiwell Holder

Room Location

  • Swann 2.16