Zeiss LSM 880 with Fast Airyscan

 

Available Laser Lines

Laser Type Line Maximum Power at x20 Objective
Diode 405nm 4.88mW
Argon 458nm NA
  488nm 2.64mW
  514nm 1.04mW
HeNe 561nm 6.82mW
HeNe 594nm 0.88mW
Diode 633nm 1.74mW

Software

  • Zen Black (on microscope)
  • Zen Black (Off line)
  • Zen Blue 2.3 (Off Line)

Available Modules

  • Fluorescence Recovery after Photobleaching (FRAP)
  • Fluorescence Correlation Spectroscopy(FCS)
  • 4D imaging (Time,X,Y and Z)
  • Definite Focus
  • Colocalisation
  • Spectral Unmixing
  • Tiles & Positions
  • XY scanning stage
  • 2 x PMT's detectors
  • 1 x GaAsp detector
  • Airyscan (120nm XY and 350nm Z)

Objectives

Type Magnification Correction NA
Plan Apochromat x10 Air 0.45
Plan Apochromat x20 Air 0.8
Plan Apochromat x40 Oil 1.3
Plan Apochromat x63 Oil 1.4
Alpha Plan Apochromat x100 Oil 1.4

Airyscan (with Fast module)

Suggested Laser Line Emission Filter
405nm 488nm BP 420-480 + BP 495-550
488nm, 561nm BP 495-550 + LP 570
561nm, 633nm BP 570-620 + LP 645
405nm, 488nm, 561nm, 594nm BP 420-480 + BP 495-620
405nm, 458nm, 488nm, 514nm BP 465-505 + LP 525
405nm, 561nm, 594nm, 633nm BP 420-480 + LP 605

Supporting Equipment

  • Environmental Chamber
  • Temperature control (Ambient - 45°C)
  • CO2 module (1-10%

Room Location

Swann 2.14