Zeiss Axio Observer

Filter Sets

Fluorophore Excitation Dichroic Emitter Filter Set
DAPI UV: 385/30nm

405

395

425/30

445/50

Zeiss Set 90 HE LED

Zeiss Set 49

CFP V: 423/44nm 450 467/24 Zeiss Set 91 HE LED
GFP/FITC/Alexa 488 B: 469/38nm

495

493

525/50

514/30

Zeiss Set 38 HE

Zeiss Set 90HE LED

YFP C: 511/44nm 538 555/25 Zeiss Set 91 LED
RFP/tdTomato G: 555/30nm

570

575

605/70

592/25

Zeiss Set 43 HE

Zeiss Set 90 HE LED

mCherry Y: 590/27nm 610 687/185 Zeiss Set 91 LED
Cy 5 R: 631/33nm 653 709/100 Zeiss Set 90 HE LED

Software

  • Zen 2.3

Available Modules

  • 4D Acquisition
  • Triggered Acquisition for high speed applications
  • Scanning Stage for multipoint acquisitions
  • Hamamatsu Orca Flash 4 V2
  • Definite Focus II

Objectives

Type Magnification Correction NA
Plan ApoChromat x40 Oil, DIC 1.4
Plan ApoChromat x60 Oil, DIC 1.4
alpha-Plan ApoChromat x100 Oil, DIC 1.46

Supporting Equipment

  • Environmental Chamber
    • Temperature Control Ambient to 45°C
    • Humidified CO2 concentration 1% to 10%

Room Location

  • Swann 2.14