Nikon Ti2 Live Imaging Microscope

Available Exciter/Emitter Combinations

Suggested Fluorophore SpectraX Illumination Available Excitation Filter Available Dichroic Available Emission Filter
DAPI 378/52 377/26 409 432/36
CFP 438/24 438/24 459 482/24
GFP 474/26 474/26 493 525/40 or 515/30
YFP 509/22 509/22 526 544/24 or 543/22
Cy3/tdTomato 554/23 543/23 573 596/83 or 595/31
TxRed/mCherry 575/25 578/21 596 641/75 or 702/197
Far Red/Cy5 635/18 638/18 652 698/70 or 730/139

Software

Nikon Elements 5.1

Available Modules

  • 4D acquisition
  • Multi-stage position
  • Triggered acquisition
  • Perfect focus for long term timelapse
  • JOBS acquisition
  • Photometrics Prime 95B

Objectives

Type Magnification Correction NA
CFI Plan Apochromat Lambda x20 Air 0.75
CFI Plan Apochromat Lambda x40 Air 0.95
CFI Plan Apochromat Lambda x60 Oil 1.3
CFI Plan Apochromat TIRF x100 Oil 1.49

Supporting Equipment

  • OKOLab Environmental chamber
  • Active CO2 Controller (1 to 11%)
  • Temperature Controller (Ambient to 45°C)

Room Location

Swann 2.10