Leica SP5 MP Confocal

Available Lasers

Laser Line Power at x20 objective
405nm 0.7mW
458nm NA
488nm 10% of maximum power 0.8mW
514nm 10% of maximum power 0.3mW
561nm 1.6mW
594nm 0.3mW
633nm 2.1mW
MP Laser 690-1020nm 3W output at 800nm (not measured)

Software

  • Leica LAS-AF (Confocal)
  • ISS Vista (FCS)
  • Becker & Hickl SPCImage (FLIM)

Available Modules

  • Conventional and Resonant Scanners
  • 5 detection channels (2xHyD)
  • Spectrometers (all visible wavelengths)
  • FRAP, FRET
  • FCS,FCCS
  • Spectral FLIM
  • 8K x 8K image size on conventional scanner
  • 25frames/sec at 512x512 using resonant scanner
  • Non-descanned external detectors for two-photon imaging

Objectives

Type Magnification Correction NA
HCX PLAN APO x20 Oil, Water, Lbd Bl 0.7
HCX PLAPO x40 Oil, PH3, CS 1.25
HCX PLAPO x63 W Corr Lbd Bl 1.2
HCX PLAPO x63 Glycerol 1.3
HCX PLAPO x63 Oil CS 1.4
HCX PLAPO x100 Oil 1.46

Supporting Equipment

  • Environmental chamber
  • Temperature control from ambient to 45°C
  • Humidified CO2 concentration 1% to 11%

Fluorescence Correlation Spectroscopy (FCS)

  • Single pixel beam positioning
  • Dual channel for Fluorescence Cross Correlation Spectroscopy (FCCS)
  • 3 Filter Sets available
    • GFP/RFP -  Band Pass filters 500-550nm and 607-683nm, Beam Splitter 560nm
    • CFP/YFP - Band Pass filters 470-500nm and 535-585nm, Beam Splitter 505nm
    • Alexa 488/633 - Band Pass filters 500-550nm and 647-703nm, Beam Splitter 625nm

Fluorescence Lifetime Imaging Microscopy (FLIM)

  • Multiphoton Laser
  • 1 internal spectral FLIM detector
  • 1 external filter based detector
  • Single pixel decay data
  • Single or multiexponential decay analysis
  • 3 filter sets for external detector
    • GFP - Band Pass 500-550nm
    • RFP - Band Pass 573-647nm
    • DAPI - Band Pass 435-485nm

Room Location

Swann 2.15